Fig. 1.

Curcumol protects mice liver from CCl₄-induced injury and ameliorates murine hepatic fibrogenesis. Mice were randomly divided into the following 5 groups (eight mice per group): vehicle control, CCl₄, CCl₄ + curcumol 15 mg kg⁻¹, CCl₄ + curcumol 30 mg kg⁻¹ and CCl₄ + curcumol 60 mg kg⁻¹. (A) Representative photograph of liver tissues, and microphotograph of H&E-stained, Masson-stained and Sirius Red-stained paraffin-embedded sections of liver tissues (Scale bars, 100 µm). (B) Determination of serum ALT, AST, ALP, LDH, TBIL and IBIL levels. (C) ELISA analysis of serum hyaluronic acid, laminin, type III procollagen, and Ⅳ collagen. (D) Western blot analysis of α-SMA, α1(I) procollagen, fibronectin and TGF-βRⅡ expression from mouse liver. (E) Immunofluorescence staining of liver sections using antibodies against EGFR, PPAR-γ. Scale bar, 100 µm. Data are expressed as mean ± SD (n = 6); *P < 0.05 versus CCl₄, **P < 0.01 versus CCl₄, and ***P < 0.001 versus CCl₄, ^##P < 0.01 versus vehicle control, ^###P < 0.001 versus vehicle control.