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. 2018 Sep 7;19:375–387. doi: 10.1016/j.redox.2018.09.007

Fig. 7.

Fig. 7

RIPK3 promotes curcumol-induced mitochondrial ROS generation and depolarization via activating JNK. (A) Human HSC-LX2 cells were treated with DMSO (0.02%, w/v), or curcumol at indicated concentrations for 24 h. Western blot analyses of the protein expression of phospho-ERK1/2, ERK1/2, phospho-JNK1/2, JNK1/2, phospho-p38, and p38. (B) Human HSC-LX2 cells were treated with 30 μM curcumol for the indicated time period. The ratio of phospho-JNK1/2 and JNK1/2 were detected by western blot analysis. (C) Human HSC-LX2 cells were pretreated with SP600125 (20 μM) for 1 h and then treated with the indicated concentration of curcumol for 24 h. Cell Counting Kit-8 assayed for evaluating cell viability. Human HSC-LX2 cells were stably transfected with RIPK3 siRNA, and then treated with the indicated concentration of curcumol for 24 h. Following the treatment, (D) the ratio of phospho-JNK1/2 and JNK1/2 was measured by western blot analysis, (E) the expression of phospho-JNK1/2 were detected by immunofluorescence. Scale bar, 50 µm. (F) MitoSox Red staining was use to evaluate mitochondrial superoxide. Scale bar, 50 µm. *P < 0.05 versus DMSO, **P < 0.01 versus DMSO, and ***P < 0.001 versus DMSO. & P < 0.05 versus control. ##P < 0.01 versus curcumol (30 μM).