Fig. S3.

Curcumol induces HSC death and suppresses CCl₄-induced cell damage in hepatocytes. Primary HSCs and hepatocytes were isolated from male ICR mice under CCl₄ and the indicated dose of curcumol treatment. (A) Trypan blue staining was used to detect cell death. Scale bar, 100 µm. (B) ROS production was assessed by DCFH-DA staining, Scale bar, 50 µm. (C) Determination of supernatant ALT, AST, LDH levels in Primary hepatocytes. Data are expressed as mean ± SD (n = 6); **P < 0.01 versus CCl₄, and ***P < 0.001 versus CCl₄.