Table 2.
Primers and temperature conditions used for PCR [29]
| Locus | Primer | Annealing temp (°C) | Cycles | Direction | Sequence (5′-3′) | Reference |
|---|---|---|---|---|---|---|
| CaM | CMD5 | 55 | 35 | Forward | CCGAGTACAAGGARGCCTTC | [53] |
| CMD6 | 55 | 35 | Reverse | CCGATRGAGGTCATRACG TGG | ||
| BenA | Bt2a | 55 | 35 | Forward | GGTAACCAAATCGGTGCTGTTC | [54] |
| Bt2b | 55 | 35 | Reverse | ACCCTCAGTGTAGTGACCCTTGGC | ||
| MAT1–1 | AFM1_F65655 MAT1_R6215 |
48 48 |
30 30 |
Forward Reverse |
CCTYGACGMGATGGGITGG TGTCAAAGARTCCAAAAGGAGG |
this study |
| MAT1–2 | MAT2_F6086 MAT2_R6580 |
48 48 |
30 30 |
Forward Reverse |
TCGACAAGATCAAAWCYCGTC CTTYTTGARCTCTTCYGCTAGG |
this study |