Fig. (2).
Growth curves showing the growth rates of MCF-7 cells when treated with 4- hydroxytamoxifen, PD98059, LY294002 and fish oil (both singly and in combination); and also estradiol. Cells were seeded at a density of 1.5 million cells per plate on day 0. Cells were counted on days 1, 2, 4, 7 and 9 and media and treatments were replenished on day 4. Cells were incubated at 37°C with 5% CO2. Cell counts represent the mean number per 3 counts (n=3 ± SD). Passage numbers were 19, 21, 24. All test solutions significantly different to control (p 0.001).
