Fig. (7).
A. Western Blot for the levels of total Src, and phosphorylated Src (at tyr 416) protein. MCF-7 and TamR cells were incubated with FO (1 μmL-1 fish oil), PD (25 µM PD98059), LY (5 µM LY294002) and 1 x 10-7 M 4-hydroxytamoxifen (MCF-7 cells only) for 3h. Cells were lysed and protein extracted and quantified for equal loading. Protein was separated by SDS-PAGE. Proteins were immobilised on a nitrocellulose membrane and immunoprobed using a cell signalling technology total Src or phospho-Src primary antibody. B. Densitometric analysis of total Src, and phosphorylated Src (at tyr 416) protein. M7- MCF-7 cells, TR- TamR cells. Densitometry was performed using Alpha Digi Doc TM RT camera and image analysis system, Genetic Research Instrumentation, Essex, UK. Passage numbers were 23, 27 and 30.
