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Western Blot for the levels of β-actin. MCF-7 and TamR cells were incubated with DMSO (0.1μml-1), FO (1μml-1 fish oil), PD (25µM PD98059), LY (5µM LY294002) and 1 x 10-7 M 4-hydroxytamoxifen (MCF-7 cells only) for 3h. Cells were lysed and protein extracted and quantified for equal loading. Proteins were separated by SDS-PAGE, immobilised on a nitrocellulose membrane and immunoprobed using β-actin primary antibody.
