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Inhibitory effect of ALE against the expression of ERK, JNK, and p38 induced by heat shock treatment in HDFs. The protein level of ERK, phospho-ERK (p-ERK), JNK, phospho-JNK (p-JNK), p38, and phospho-p38 (p-p38) was determined by western blot. Each of the figures was representative of three independent experiments. Beta-actin (β-actin) was used as an internal control to confirm equal protein loading.
