Table 1.
Amino acid sequence identities/similarities of putative proteins encoded by the pE35048-oc (GenBank accession no. MF580438).
| BLASTP
analysisa |
|||||||
|---|---|---|---|---|---|---|---|
| ORF | Start (bp) | Stop (bp) | Size (amino Acid) | Predicted function | Most significant database match | Accession no. | % Amino acid identity (% aminacid similarity) |
| orfl | 1833 | 1 | 610 | DNA Topoisomerase III | Type 1 topoisomerase (plasmid) [Enterococcus faecium] | YP_976069.1 | 100 (100) |
| orfl | 3,818 | 1,932 | 628 | Group II intron | Group II intron reverse transcriptase/maturase [Lactobacillales] | WP_010718345.1 | 100 (100) |
| bΔorf3 | 4,917 | 4,555 | 120 | DNA Topoisomerase III | Topoisomerase [Bacilli] | WP_000108744.1 | 100 (100) |
| orf4 | 5,534 | 4,917 | 205 | Resolvase | Resolvase (plasmid) [E. faecalis] | YP_003864109.1 | 99 (100) |
| orf5 | 5,718 | 5,548 | 56 | Hypothetical protein pRE25p07 (plasmid) [E. faecalis] | YP_783891.1 | 98 (100) | |
| orf6 | 7,557 | 6,067 | 496 | Replication protein | Replication protein (plasmid) [E. faecium] | NP_044463.1 | 100 (100) |
| orf7 | 8,213 | 7,941 | 90 | Transcriptional regulator | CopS (plasmid) [Streptococcus pyogenes] | YP_232751.1 | 100 (100) |
| Δorf8 | 8,901 | 8,446 | 151 | Responsible for lincomycin resistance | Lincosamide nucleotidyltransferase (plasmid) [E. faecium] | ARQ19308.1 | 99 (100) |
| orf9 | 9,772 | 8,873 | 299 | Transposase | Transposase [Streptococcus suis] | AGO02197.1 | 100 (100) |
| orf10 | 10,443 | 9,769 | 224 | Transposase | IS3 family transposase [E. faecalis] | WP_013330754.1 | 100 (100) |
| orf11 | 11,867 | 10,809 | 352 | 23S ribosomal RNA methyltransferase | Cfr family 23S ribosomal RNA methyltransferase [Staphylococcus aureus] | WP_001835153.1 | 100 (100) |
| orf12 | 13,177 | 12,278 | 299 | Transposase | Transposase [S. suis] | AGO02197.1 | 100 (100) |
| orf13 | 13,848 | 13,174 | 224 | Transposase | IS3 family transposase [E. faecalis] | WP_013330754.1 | 100 (100) |
| Δorf8 | 13,921 | 14,061 | 47 | Responsible for lincomycin resistance | Lincomycin resistance protein [synthetic construct] | AGT57825.1 | 100 (100) |
| orf14 | 15,391 | 14,531 | 289 | Hypothetical protein [S. suis] | WP_079268203.1 | 96 (98) | |
| orf15 | 15,822 | 15, 451 | 123 | Hypothetical protein [Enterococcus casseliflavus] | WP_032495652.1 | 99 (99) | |
| orf16 | 16,546 | 15,809 | 245 | Hypothetical protein [E. faecalis] | WP_012858057.1 | 100 (100) | |
| orf17 | 17,768 | 16,836 | 310 | Hypothetical protein [Enterococcus sp. HMSC063D12] | WP_070544061.1 | 100 (100) | |
| orf18 | 18,693 | 17,770 | 307 | Membrane protein insertase | Hypothetical protein [Enterococcus sp. HMSC063D12] | WP_070544063.1 | 99 (99) |
| orf19 | 20,366 | 18,711 | 551 | Type IV secretory pathway, VirD4 component, TraG/TraD family ATPase | Hypothetical protein [Enterococcus] | WP_002325630.1 | 100 (100) |
| orf20 | 20,790 | 20,359 | 143 | Ypsilon (plasmid) [E. faecalis] | YP_003864141.1 | 100 (100) | |
| orf21 | 21,346 | 20,795 | 183 | Hypothetical protein [E. faecium] | WP_02 9485693.1 | 99 (99) | |
| orf22 | 22,468 | 21,359 | 369 | Amidase | Putative lytic transglycosylase (plasmid) [E. faecalis] | YP_003864139.1 | 99 (99) |
| orf23 | 23,842 | 22,490 | 450 | Conjugal transfer protein TraF [E. faecium] | WP_085837474.1 | 98 (99) | |
| orf24 | 25,817 | 23,856 | 653 | Type IV secretory pathway, VirB4 component | TrsE (plasmid) [E. faecalis] | YP_003864137.1 | 100 (100) |
| orf25 | 26,457 | 25,828 | 209 | Hypothetical protein [Enterococcus] | WP_002325627.1 | 99 (100) | |
| orf26 | 26,857 | 26,474 | 127 | AM21 (plasmid) [E. faecalis] | YP 003305365.1 | 100 (100) | |
| orf27 | 27,208 | 26,876 | 110 | T4SS_CagC | Hypothetical protein pRE25p25 (plasmid) [E. faecalis] | YP_783909.1 | 100 (100) |
| orf28 | 29,217 | 27,232 | 661 | Nickase | Molybdopterin-guanine dinucleotide biosynthesis protein MobA [E. faecalis] | WP_025186512.1 | 99 (100) |
| orf29 | 29,509 | 29,808 | 99 | Hypothetical protein pRE25p23 (plasmid) [E. faecalis] | YP_783907.1 | 100 (100) | |
| orf30 | 29,811 | 30,068 | 85 | Hypothetical protein [Enterococcus] | WP_021109234.1 | 100 (100) | |
| orf31 | 30,927 | 30,430 | 165 | Molecular chaperone DnaJ | Molecular chaperone DnaJ [Enterococcus] | WP_025481726.1 | 97 (98) |
| orf32 | 31,353 | 30,946 | 135 | Hypothetical protein pRE25p20 (plasmid) [E. faecalis] | YP_783904.1 | 98 (99) | |
| Δorf33 | 32,376 | 31,705 | 223 | Zeta-toxin | Toxin zeta [E. faecium] | WP_002300569.1 | 97 (98) |
| orf34 | 33,173 | 32,412 | 253 | DNA replication protein DnaC | AAA family ATPase [Proteiniborus ethanoligenes] | WP_091728780.1 | 94 (98) |
| orf35 | 34,750 | 33,170 | 526 | ISEfa15 transposase | Transposase [P. ethanoligenes] | WP_091728892.1 | 70 (84) |
| orf36 | 36,973 | 35,006 | 655 | ABC-F type ribosomal protection protein | ABC-F type ribosomal protection protein OptrA [E. faecalis] | WP_078122475.1 | 97 (98) |
| orf37 | 38,100 | 37,078 | 340 | ISEfa15 transposase (partial) | Transposase [Clostridium formicaceticum] | WP_070963420.1 | 64 (80) |
| Δorf33 | 38,423 | 39,199 | 75 | Zeta-toxin | Zeta toxin [E. faecium] | WP_080440976.1 | 100 (100) |
| orf38 | 38,697 | 38,425 | 90 | Epsilon-antitoxin | Antidote of epsilon-zeta post-segregational killing system (plasmid) [S. pyogenes] | YP_232758.1 | 100 (100) |
| orf39 | 38,929 | 38,714 | 71 | Omega-repressor | Transcriptional repressor (plasmid) [S. pyogenes] | YP_232757.1 | 99 (100) |
| orf40 | 39,917 | 39,021 | 298 | ParA putative ATPase | Chromosome partitioning protein ParA [S. suis] | WP_0023 87620.1 | 100 (100) |
| orf41 | 40,445 | 40,314 | 43 | Hypothetical protein (plasmid) [Pediococcus acidilactici] | WP_002321978.1 | 100 (100) | |
| orf42 | 41,187 | 40,450 | 245 | 23S rRNA (adenine(2058)-N(6)) methyltransferase | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) [S. suis] | WP_024418925.1 | 99 (100) |
aFor each ORF, only the most significant identity detected is listed. bΔ represents a truncated ORF.