Table 2.
Virus, gene, amplicon, and primer sequences used in the present study.
| Virus | Gene | Amplicon | Primers | Sequence | Reference |
|---|---|---|---|---|---|
| Avian Nephritis Virus | ORF 1 | 473 bp | ANV Pol 1F | 5΄-GYTGGGCGCYTCYTTTGAYAC-3΄ | Day et al., 2007 |
| ANV Pol 1R | 5΄-CRTTTGCCCKRTARTCTTTRT-3΄ | ||||
| UTR | 182 bp | ANV F | 5΄-ACG GCG AGT ACC ATC GAG-3΄ | Todd et al., 2010 | |
| ANV R | 5΄-AAT GAA AAG CCC ACT TTC GG-3΄ | ||||
| Chicken Astrovirus | ORF 1b | 362 bp | Cas Pol 1F | 5΄-GAYCARCGAATGCGRAGRTTG-3΄ | Day et al., 2007 |
| Cas Pol 1R | 5΄-TCAGTGGAAGTGGGKARTCTAC-3΄ | ||||
| Chicken Parvovirus | NS | 561 bp | PV 1F | 5΄-TTCTAATAACGATATCACT-3΄ | Zsak et al., 2009 |
| PV 1R | 5΄-TTTGCGCTTGCGGTGAAGTCTGGCTCG-3΄ | ||||
| Infectious Bronchitis Virus | UTR | 179 bp | UTR 11 | 5΄-ATGTCTATCGCCAGGGAAATGTC-3΄ | Cavanagh et al., 2002 |
| UTR 41 | 5΄-GGGCGTCCAAGTGCTGTACCC-3΄ | ||||
| UTR 31 | 5΄-GCTCTAACTCTATACTAGCCTA-3΄ | ||||
| Avian Rotavirus | NSP4 | 630 bp | NSP4-F30 | 5΄-GTGCGGAAAGATGGAGAAC-3΄ | Day et al., 2007 |
| NSP4-R660 | 5΄-GTTGGGGTACCAGGGATTAA-3΄ | ||||
| Fowl Adenovirus-1 | HEXON | 897 bp | Hexon A | 5΄-CAARTTCAGRCAGACGGT-3΄ | Meulemans et al., 2001 |
| Hexon B | 5΄-TAGTGATGMCGSGACATCAT-3΄ | ||||
| Avian Reovirus | S4 | 1120 bp | S4-F13 | 5΄-GTGCGTGTTGGAGTTTCCCG-3΄ | Pantin-Jackwood et al., 2008 |
| S4-1133 | 5΄-TACGCCATCCTAGCTGGA-3΄ | ||||
| Newcastle Diseases virus | F | 324 bp | NEW S | 5΄-GGAGGATGTTGGCAGCATT-3΄ | Pang et al., 2002 |
| NEW AS |
bp = base pair.