Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 18;5:180191. doi: 10.1038/sdata.2018.191

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.

PMC Copyright notice

(a) Exemplary image of stained lysosomes in a fixed sample. Scale bar: 2 μm (b) First frame of a stack of images of stained lysosomes in a living cell, acquired with the appropriate parameters. Temporal resolution: 65 ms/frame. Scale bar: 2 μm (c) First frame of a stack of images of stained lysosomes in a living cell, acquired at low speed: artifactual deformation of the apparent lysosome size due to organell motion during imaging is clearly visible. Temporal resolution: 10 s/frame. Scale bar: 2 μm. (d) Example of size calculation for imaged lysosomes in blue ROI of (a), (b) and (c). The intensity profile along the blue line was fitted with a Gaussian function to retrieve the FWHM, i.e. an estimate of spot size. FWHM values are reported for each fitting. (e) Graphical representation of size values obtained by image analysis described in panel (d) for all imaged lysosomes (black square, mean value and standard deviation), for lysosomes enclosed within the blue ROI (blue triangle) and retrieved by iMSD analysis (red circle).