ESE-1 knockdown inhibits transformation properties in trastuzumab resistant HER2+ cell lines. A, B. Stable ESE-1 knockdown inhibits proliferation in trastuzumab resistant HER2+ cell lines BT474-HR20 and SKBR3-Pool2 cell line. Proliferation was measured via trypan blue exclusion method by Vicell. C, D, E, F. Downregulation of ESE-1 inhibits clonogenicity, and soft agar colony formation in the two resistant sublines. Data represented is cumulative of three independent replicates. Error bars represent mean +/− SEM derived from three independent experiments.