MmCobD compensates for the absence of PduX in a S. enterica pduX strain in vivo. Cobamide-dependent growth was assessed with S. enterica cells grown aerobically at 37°C in NCE minimal medium with (A) glycerol (22 mM) and (CN)2Cby (1 nM) or (B) ethanolamine (90 mM) and (CN)2Cby (300 nM), supplemented with, DMB (0.15 mM), ampicillin (0.1 mg mL−1), L(+)-arabinose (0.5 mM) and MgSO4 (1 mM). S. enterica pduX+ and ΔpduX strains with plasmids encoding MmCobD, MmCobD1-385, MmCobD386-497, SePduX, or pBAD24 as the empty vector control. Growth analyses were repeated in three independent experiments with the error bars representing the standard error mean of cultures grown in triplicate.