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Clonogenic analysis of four CRC cell lines exposed to ENMD-2076. (a) HCT116, (b) GEO, (c) LS1034, (d) LS123 were plated in six-well plated and exposed to increasing concentrations of ENMD-2076 (0.1, 0.5, 1, and 2 µmol/l) for 72 h. Drug was removed and replaced with media to allow for regrowth of clones. Cells were stained with crystal violet, photographed, and quantitated using Image J software using the colony area plugin.
