Fig 5. Retention of telomerase activity in ATRX^KO clones that display ALT-associated hallmarks.

(A) TRAP analysis shows retention of telomerase activity in all EV and ATRX^KO clones from U-251 and UW479. NTC indicates no template control. Parental U-251 and UW479 lysates were RNase treated as specificity controls. (B) Quantification of TRAP signals reveals clonal variability in telomerase activity within EV and ATRX^KO clones from U-251 and UW479. Mean telomerase activity levels are shown from two independent experiments, and error bars represent standard error of the mean.