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. 2018 Sep 18;13(9):e0204159. doi: 10.1371/journal.pone.0204159

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© 2018 Brosnan-Cashman et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — ATRX knockdown using 3 different anti-ATRX shRNA lentiviral constructs in MOG-G-UVW, U-251, SF188, and UW479 was confirmed using (A) immunohistochemistry and (B) immunoblotting against ATRX. Arrowheads indicate the band representing full-length, wild-type ATRX. Empty lentiviral vector (pLKO.1) serves as a non-knockdown control for ATRX expression.