Fig 2. DNA methylation profiles (mean % ± S.D.) of barramundi Lates calcarifer sex-related genes within testes (n = 10) and ovaries (n = 4) of wild caught individuals and testes (n = 14) and ovaries (n = 14) of captive broodstock, as obtained by bisulfite amplicon next-generation sequencing.

(a) female-biased methylated genes dmrt1 and nr5a2; (b) male-biased methylated genes cyp19a1 and amh. Within each CpG site, different letters denote significant differences between groups (P < 0.05).