Table 3. Differential methylation patterns (mean percentage ± S.D.) within sex related genes targeted by bisulfite amplicon next-generation sequencing among Lates calcarifer adults of captive and wild origin.
Different letters denote significant differences among groups (P < 0.01).
| Gene (amplicon #) |
Amplicon size (position from TSS) |
# CpG Per amplicon |
Testes (%) | Ovaries (%) | Overall (%) | ||
|---|---|---|---|---|---|---|---|
| Captive | Wild | Captive | Wild | ||||
| dmrt1 (1) | 270 bp (-575, -305) | 5 | 41.2 ± 17.1 a | 67.8 ± 20.2 ab | 58.6 ± 14.6 ab | 71.0 ± 12.3 b | 59.6 ± 19.1 |
| dmrt1 (2) | 428 bp (-255, +173) | 17 | 6.8 ± 6.9 a | 6.5 ± 7.3 a | 17.0 ± 9.5 b | 16.3 ± 10.4 b | 11.6 ± 9.8 |
| nr5a2 (1) | 302 bp (-450, -148) | 10 | 10.2 ± 1.3 a | 10.7 ± 1.4 ac | 16.2 ± 4.8 bc | 20.6 ± 4.4 b | 14.4 ± 5.4 |
| nr5a2 (2) | 194 bp (-79, +115) | 8 | 12.9 ± 2.2 a | 13.4 ± 1.1 a | 59.3 ± 12.4 b | 59.3 ± 12.9 b | 36.2 ± 24.9 |
| cyp19a1 | 267 bp (-107, +160) | 8 | 92.5 ± 2.0 a | 88.0 ± 8.2 a | 44.1 ± 8.7 b | 54.0 ± 8.9 b | 69.6 ± 22.4 |
| Amh | 341 bp (-258, +83) | 6 | 94.0 ± 1.4 a | 76.6 ± 3.0 b | 59.5 ± 2.2 c | 46.0 ± 2.7 d | 69.0 ± 18.5 |
| foxl2 (1) | 255 bp (-282, -27) | 9 | 3.7 ± 0.7 a | 6.9 ± 0.6 b | 5.8 ± 0.8 bc | 4.0 ± 0.7 ac | 5.1 ± 1.5 |
| foxl2 (2) | 308 bp (+374, +682) | 18 | 6.4 ± 0.9 a | 8.61 ± 0.9 b | 7.55 ± 1.1 ac | 8.5 ± 1.0 bc | 7.8 ± 1.3 |
| sox8 (1) | 368 bp(-195, +173) | 26 | 1.5 ± 1.2 | 2.0 ± 1.0 | 2.3 ± 1.3 | 2.4 ± 2.6 | 2.0 ± 1.7 |
| sox8 (2) | 353 bp (+151, +504) | 20 | 7.1 ± 1.2 a | 10.0 ± 1.7 b | 9.1 ± 1.5 b | 10.5 ± 2.5 b | 9.2 ± 2.2 |
| sox9 | 344 bp (-130, +214) | 19 | 0.8 ± 0.8 | 1.1 ± 0.9 | 1.1 ± 0.7 | 1.3 ± 0.7 | 1.1 ± 0.8 |