. 2018 Sep 6;14(9):e1007611. doi: 10.1371/journal.pgen.1007611

Table 1. Test of effect of the Pde4b genotype on the number of adenomas in the colon of Apc ^Min/+ mice over a series of backcross-intercross generations.

Gross tumor numbers were measured in the colons of Apc^Min/+ mice from the series of backcross-intercross generations, carrying the Pde4b^+/+, Pde4b^+/- and Pde4b^-/- genotypes. Pde4b^+/+ animals (110) show the lowest average colon tumor count 2.4 +/- 2.2. Pde4b^+/- animals (192) show higher average colon tumor counts than Pde4b^+/+ animals 3.3 ± 2.9. Finally Pde4b^-/- mice (66) show the highest average colon tumor number 4.3 ± 4.6. p = 0.0004 by the Kruskal-Wallis Test of association between tumor number and number of mutant alleles of Pde4b. We reject the null hypothesis that there is no difference in gross colonic tumor number as a function of Pde4b genotype.

Genotype	Colon tumor counts, mean ± SD (n of mice)
Genotype	Apc^Min/+ Pde4b^+/+	Apc^Min/+ Pde4b^+/-	Apc^Min/+ Pde4b^-/-
F2	2.4 ± 1.5 (9)	3.1 ± 2.9 (18)	4.0 ± 2.2 (7)
N2F2	2.6 ± 2.4 (69)	3.1 ± 2.9 (124)	4.2 ± 5.2 (39)
N3F2	2.4 ± 2.1 (23)	4.2 ± 3.2 (33)	5.1 ± 4.3 (10)
N4F2	2.1 ± 2.3 (9)	2.9 ± 1.7 (17)	4.0 ± 3.7 (10)

Table 1. Test of effect of the Pde4b genotype on the number of adenomas in the colon of Apc Min/+ mice over a series of backcross-intercross generations.

Table 1. Test of effect of the Pde4b genotype on the number of adenomas in the colon of Apc ^Min/+ mice over a series of backcross-intercross generations.