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. 2018 Sep 6;14(9):e1007658. doi: 10.1371/journal.pgen.1007658

Fig 4. An independent CRISPR/Cas9 induced Plcb4 allele validates the rescue phenotype.

Fig 4

A) The CRISPR/Cas9-induced Plcb4ins1 allele (insertion of the nucleotide ‘A’ at amino acid 328) results in a frameshift to the protein coding sequence leading to a premature stop codon. B) Sanger sequencing analysis of gDNA from a wildtype mouse and both gDNA and cDNA from a Plcb4+/ins1 mouse at the Plcb4 1bp insertion site. C) 169 progeny genotyped from a validation cross of F5L/L Plcb4+/ins1 mice with F5L/+ Tfpi+/-.