Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (E. coli) |
S17-1 lambda pir | PMID_6340113 |
thi pro hdsR hdsM + recA, chromosomal insertion of RP4-2(Tc::Mu Km::Tn7), AmpS |
|
| Strain, strain background (E. coli) |
BL21 Rosetta (DE3) | Novagen | F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR) |
|
| Strain, strain background (Bacteroides thetaiotaomicron) |
VPI-5482 ∆tdk | PMID_18611383 | ||
| Strain, strain background (B. thetaiotaomicron) |
VPI-5482 ∆tdk ∆locus1 ∆locus3 | PMID_24439897 | ||
| Strain, strain background (B. thetaiotaomicron) |
VPI-5482 ∆tdk ∆locus1 ∆btuG2 ∆locus3 | This paper | ||
| Strain, strain background (B. thetaiotaomicron) |
VPI-5482 ∆tdk ∆locus1 ∆btuB2 ∆locus3 | PMID_24439897 | ||
| Strain, strain background (B. thetaiotaomicron) |
VPI-5482 ∆tdk ∆locus1 ∆locus2 ∆locus3 | PMID_24439897 | ||
| Strain, strain background (B. thetaiotaomicron) |
VPI-5482 ∆tdk ∆locus1 ∆locus3 att::pNBU2_tet_BC01 | This paper | ||
| Strain, strain background (B. thetaiotaomicron) |
VPI-5482 ∆tdk ∆locus1 ∆btuG2 ∆locus3 att::pNBU2_tet_BC14 | This paper | ||
| Strain, strain background (B. thetaiotaomicron) |
VPI-5482 ∆tdk ∆locus1 ∆btuB2 ∆locus3 att::pNBU2_tet_BC14 | This paper | ||
| Strain, strain background (B. thetaiotaomicron) |
VPI-5482 ∆tdk ∆locus1 ∆btuG2 ∆locus3 att::pNBU2_tet_BC16_us1957_btuG2 | This paper | ||
| Strain, strain background (E. coli) |
BL21 Rosetta (DE3)
pET21_NESG_btuG2 |
This paper | ||
| Strain, strain background (E. coli) |
BL21 Rosetta (DE3) pET21_NESG_btuG2_10xHis | This paper | ||
| Strain, strain background (E. coli) |
BL21 Rosetta (DE3) pET21_NESG_BVU2056 | This paper | ||
| Strain, strain background (E. coli) |
BL21 Rosetta (DE3) pET21_NESG_BACUNI04578 | This paper | ||
| Strain, strain background (E. coli) |
BL21 Rosetta (DE3) pET21_NESG_BACCOPRO02032 | This paper | ||
| Recombinant DNA reagent |
pExchange-tdk | PMID: 18611383 | plasmid | |
| Recombinant DNA reagent |
pExchange_tdk_∆btuG2 | plasmid | ||
| Recombinant DNA reagent |
pNBU2_ermG | PMID: 18611383 | plasmid | |
| Recombinant DNA reagent |
pNBU2_ermG_us1957 | This paper | plasmid | |
| Recombinant DNA reagent |
pNBU2_tet_BC01 | PMID: 18996345 | plasmid | |
| Recombinant DNA reagent |
pNBU2_tet_BC14 | PMID: 18996345 | plasmid | |
| Recombinant DNA reagent |
pNBU2_tet_BC16 | PMID: 24439897 | plasmid | |
| Recombinant DNA reagent |
pNBU2_erm_us1957_btuG2_C32A | This paper | plasmid | |
| Recombinant DNA reagent |
pNBU2_erm_us1957_btuG2_∆ss | This paper | plasmid | |
| Recombinant DNA reagent |
pNBU2_erm_us1957 _btuG2_K34A W35A D36A |
This paper | plasmid | |
| Recombinant DNA reagent |
pNBU2_erm_us1957_btuG2_susD-ss | This paper | plasmid | |
| Recombinant DNA reagent |
pNBU2_erm_us1957_btuG2_susD-ss-LES | This paper | plasmid | |
| Recombinant DNA reagent |
pNBU2_erm_us1957_btuB2_FLAG_HA | This paper | plasmid | |
| Recombinant DNA reagent |
pNBU2_erm_us1957 _BT1763_FLAG_HA |
This paper | plasmid | |
| Recombinant DNA reagent |
pNBU2_erm_us1957 _BT3704_HA |
This paper | plasmid | |
| Recombinant DNA reagent |
pET21_NESG_btuG2 | Northeast Structural Genomics Consortium; PDB 3DSM |
plasmid | |
| Recombinant DNA reagent |
pET21_NESG_btuG2_10xHis | This paper | plasmid | |
| Recombinant DNA reagent |
pET21_NESG_BVU2056 | This paper | plasmid | |
| Recombinant DNA reagent |
pET21_NESG_ BACUNI04578 |
This paper | plasmid | |
| Recombinant DNA reagent |
pET21_NESG_ BACCOPRO02032 |
This paper | plasmid |