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. 2018 Sep 3;7:e37429. doi: 10.7554/eLife.37429

Figure 4. FGF signalling in HH24g mesenchyme.

Figure 4.

(a-e) Resetting of gene expression in HH24g mesenchyme. Expression levels of Dusp6 (a), Spry2 (c) and Fgf18 (e) determined by RNA-seq. (b) In situ hybridization showing equivalent levels of Dusp6 (n = 3/3, (b), Spry2 (n = 3/3, (d), and Fgf18 (n = 3/3, (f) in HH24 and contralateral HH24g mesenchyme. (g–j) FGF-soaked beads (j) n = 3/3), but not PBS-soaked beads (h) n = 3/3), up-regulate expression of Dusp6 after 6 hr (control left wing buds flipped horizontally – (g) and (i). (k) Flow cytometry of wing bud distal mesenchyme: PBS-soaked beads do not significantly affect proportion of cells in G1-phase after 6 hr compared to left wing controls (Pearson’s χ2 test – p=0.5); FGF-soaked beads implanted at HH27 significantly increase G1-phase cells compared to left wing control after 6 hr (Pearson’s χ2 test - p<0.0001), note boxes indicate separate experiments. Scale bars: HH24 buds, HH27 buds - 300 μm; HH29 buds - 200 μm.

Figure 4—source data 1. Flow cytometry graphs for cell cycle analyses.
elife-37429-fig4-data1.pdf (399.4KB, pdf)
DOI: 10.7554/eLife.37429.010