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. 2018 Sep 18;7:e38161. doi: 10.7554/eLife.38161

Figure 1. Hypocotyl epidermal cells exhibit a wave of growth in length and not in width.

(A) A scanning electron micrograph of a 24HPG etiolated hypocotyl showing cell indices in a non-dividing cell file, numbered from the collet to the cotyledons. (B) Cell length and width by cell position index displayed from 0-54HPG. (C) Cell shape anisotropy (length:width) by cell index from 0-48HPG. (D) Relative growth rates (RGR) for length and width of cells by index from 0-66HPG, in 6H windows. Data in B-D were collected from 20 hypocotyls per time point imaged by confocal microscopy, from at least two non-dividing files per hypocotyl. The data in B are presented without error bars to make visualization possible; standard errors can be found in Figure 1—figure supplement 1. RGR values and standard errors can be found in Supplementary file 1. Blue arrowheads point to early-pulse growth adjacent to the collet, red arrowheads to early growth suppression, light blue arrowheads trace the maximal RGR(L) and proxy the acropetal wave.

Figure 1.

Figure 1—figure supplement 1. Characterization of dividing and non-dividing cell files and growth in non-dividing files.

Figure 1—figure supplement 1.

(A) cell number in dividing cell files from 0HPG to 24HPG. (B) GL2::GFP expression at 24HPG marking non-dividing cells. Dividing cell files are marked with asterisks. Scale bar = 50 µm. (C) Cell length by position index from 0HPG to 72HPG, including standard error of the means. (D) Cell width by position index from 0HPG to 72HPG, including standard error of the means.