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. 2018 Sep 18;7:e38161. doi: 10.7554/eLife.38161

Figure 2. Microtubule alignment is weakly transversely aligned early in hypocotyl growth.

(A) Representative images of microtubule organization as visualized with 35S::GFP-MAP4 at 0HPG, 24HPG and 65HPG. Scale bars = 10 µm. Location of images reported as: outer or inner epidermal face, or cortex. Dotted outlines in cortex image indicate cortex cell outlines. (B) Frequency distribution of microtubule angle grouped in 10 degree intervals from 0HPG to 65HPG using MicroFilament Analyzer (MFA); sample numbers were: 0HPG: n = 65 cells (from 5 hypocotyls); 24HPG: n = 30 (from 9 hypocotyls); 65HPG: n = 13 (from 6 hypocotyls). For 24HPG cortex analysis, n = 36 (from 5 hypocotyls). Examples of MTs at 24HPG outer epidermal faces visualized with 35S::GFP-TUA6, 35S::GFP-EB1 and CESA3::CESA3-GFP are found in Figure 2—figure supplement 1.

Figure 2.

Figure 2—figure supplement 1. A wider selection of MT markers and CESA3 used for verification.

Figure 2—figure supplement 1.

(A) Microtubule markers and a cellulose synthase marker imaged from bottom cells at 24HPG; outer epidermal face. The markers show similar patterns in microtubule alignment, that is little alignment, to the GFP-MAP4 images in Figure 2. Both EB1 and CESA images are temporal color-coded projections of an xytz acquisition at the base of the hypocotyl with 10 stacks over 4 min 41 s for EB1 and 40 stacks over 9 min 49 s for CESA3; these are dynamic markers. Scale bars = 10 µm.