Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (C. elegans) |
cpIs89[Pwrt-2>2x mTurq2::PH::tbb-2 3'UTR loxN] I; cpIs85[Pegl-20 > 2 x mKate2::PH::3xHA::tbb-2 3'UTR loxN] II; egl-20(cp221[egl-20::m NG^3xFlag]) IV |
This paper | LP515 | |
| Strain, strain background (C. elegans) |
egl-20(cp221[egl-20::mNG^3xFlag]) IV; qyIs541[Pmyo-3>mCherry::P H::tbb-2 3'UTR] |
This paper | LP673 | |
| Strain, strain background (C. elegans) |
cpIs128[Pwrt2>2x mTurquoise2 ::PH::3xHA::tbb-2 3'UTR loxN] I; mig-1(cp360[mig-1::mKate2^ 3xMyc]) I; egl-20(cp221[egl-20: :mNG^3xFlag]) IV |
This paper | LP727 | |
| Strain, strain background (C. elegans) |
cpIs89[Pwrt-2>2x mTurquoise2 ::PH::tbb-2 3'UTR loxN] I; mig -5(cp385[mNG-GLO^AID::mig-5]) II |
Heppert et al., 2018 | LP728 | |
| Strain, strain background (C. elegans) |
cpIs92 [Pmec-7>2x mTurq2::PH:: 3xHA::tbb-2 3'UTR loxN] I; cpIs129 [Pgcy-32 > 2 x mKate2:: PH::3xHA::tbb-2 3'UTR loxN] II; egl-20(cp353[egl-20::mNG^3xFlag]) IV |
This paper | LP729 | |
| Strain, strain background (C. elegans) |
cpIs92 [Pmec-7>2x mTurq2::PH: :3xHA::tbb-2 3'UTR loxN] I; cpIs129 [Pgcy-32 > 2 x mKate2::PH ::3xHA::tbb-2 3'UTR loxN] II |
This paper | LP730 | |
| Strain, strain background (C. elegans) |
cpIs130[Pwrt-2>2x mKate2 ::PH::3xHA::let-858 3'UTR::Ptag-168 > HisCl1::tbb-2 3'UTR loxN] II; egl-20 (cp353[egl-20::mNG^3xFlag]) IV |
This paper | LP732 | |
| Strain, strain background (C. elegans) |
cpIs130[Pwrt-2>2x mKate2::PH:: 3xHA::let-858 3'UTR::Ptag-168 > HisCl1::tbb-2 3'UTR loxN] II; egl-20(cp400[egl-20::YPET^3xFlag]) IV |
This paper | LP783 | |
| Strain, strain background (C. elegans) |
cpIs156[Pwrt2>2x mTurquoise2::PH: :3xHA::tbb-2 3'UTR SEC + loxN] I; lin-1 7(cp391[lin-17::mScarlet-C1^AID]) I; egl-20(cp221[egl-20::mNG^3xFlag]) IV |
This paper | LP790 | |
| Strain, strain background (C. elegans) |
mig-1(cp360[mig-1::mKate2^3xMyc]) I; lin-17(cp404[lin-17::mTurquoise2^AID]) I; egl-20(cp221[egl-20::mNG^3xFlag]) IV |
This paper | LP792 | |
| Strain, strain background (C. elegans) |
cpIs92 [Pmec-7>2x mTurq2:: PH::3xHA::tbb-2 3'UTR loxN] I; cpIs129 [Pgcy-32 > 2 x mKate 2::PH::3xHA::tbb-2 3'UTR loxN] II; egl-20(n585) IV |
This paper, CGC | LP793 | egl-20(n585) crossed to LP730 |
| Strain, strain background (C. elegans) |
cpIs92 [Pmec-7>2x mTurq2:: PH::3xHA::tbb-2 3'UTR loxN] I; cpIs129 [Pgcy-32 > 2 x mKate2 ::PH::3xHA::tbb-2 3'UTR loxN] II; egl-20(cp400[egl-20:: YPET^3xFlag]) IV |
This paper | LP795 | |
| Strain, strain background (C. elegans) |
cpIs117[Peft-3::FRT > 2 x mKate2::PH::let-858 3'UTR::FRT > 2 x mTurquoise2::PH::3x HA::tbb-2 3'UTR + loxN] I; egl-20(cp411[flp::F2A::egl-20]) IV; egl-20(cp221[egl-20::m NG^3xFlag]) IV; |
This paper | LP805 | |
| Strain, strain background (C. elegans) |
cpIs158[Pmyo-3>pat-3sp::2x vhhGFP4:: CD8 tm::2x mTurquoise2::PH::tbb-2 3'UTR loxN] I; cpIs130[Pwrt-2>2x mKate2::PH::3xHA::let-858 3'UTR ::Ptag-168 > HisCl1::tbb-2 3'UTR loxN] II; egl-20(cp400 [egl-20::YPET^3xFlag]) IV |
This paper | LP815 | |
| Strain, strain background (C. elegans) |
cpIs159[egl-5(K enhancer)::pes-10 delta > flp::SL2::2x mTurq2::PH::3xHA::tbb-2 3'UTR loxN] I; egl-20(cp413[egl-20::FRT5T2:: mKate2::let-858 3'UTR::FRT5T2:: mNG^3xFlag]) IV |
This paper | LP817 | |
| Strain, strain background (C. elegans) |
cpIs160 [Plin-44 > egl-20::mNG ::SL2::2x mTurq2::PH::3x HA::tbb-2 3'UTR loxN] I; cpIs130[Pwrt-2>2x mKate2::PH::3xHA::let-858 3'UTR::Ptag-168 > HisCl1:: tbb-2 3'UTR loxN] II |
This paper | LP818 | |
| Recombinant DNA reagent |
Peft-3>Cas9+PU6>empty sgRNA | Dickinson et al., 2013 | pDD162 | vector for Cas9 + sgRNA cloning |
| Recombinant DNA reagent |
Peft-3>Cas9+ttTi5605 sgRNA | Dickinson et al., 2013 | pDD122 | Cas9 + sgRNA targeting genomic site near ttTi5605 |
| Recombinant DNA reagent |
Peft-3>Cas9+ttTi4348 sgRNA | This paper | pAP082 | Cas9 + sgRNA targeting genomic site near ttTi4348. Derived from pDD122. |
| Recombinant DNA reagent |
empty promoter > 2 x mKate2::PH::3xHA::tbb-2 3'UTR loxN SEC loxN ttTi5605 |
This paper | pAP087.2 | vector for plasma membrane reporter insertions near ttTi5605. Derived from pCFJ150. |
| Recombinant DNA reagent |
empty promoter > 2 x mTurq2::PH::3xHA::tbb-2 3'UTR loxN SEC loxN ttTi4348 |
This paper | pAP088 | vector for plasma membrane reporter insertions near ttTi4348. Derived from pCFJ352. |
| Recombinant DNA reagent |
mNG^SEC^3xFlag | Dickinson et al., 2015 | pDD268 | vector for cloning homologous repair templates |
| Recombinant DNA reagent |
YPET^SEC^3xFlag | Dickinson et al., 2015 | pDD283 | vector for cloning homologous repair templates |
| Recombinant DNA reagent |
mKate2^SEC^3xMyc | Dickinson et al., 2015 | pDD287 | vector for cloning homologous repair templates |