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. Author manuscript; available in PMC: 2018 Sep 18.
Published in final edited form as: Mol Cell Endocrinol. 2018 Feb 3;473:166–177. doi: 10.1016/j.mce.2018.01.016

Fig. 5.

Fig. 5.

HEK293-T cells overexpressing WT-STAT3 and variants were examined immunohistochemically for p-STAT3 (tyr705) detection under unstimulated conditions (upper panels) and after 2 h-GH treatment (200 ng/mL, lower panels). Number of positive p-STAT3 nuclei were normalized to total cells and expressed as Mean ± SEM from at least six random microscopic field images of two independent experiments. Mann-Whitney test (*P < 0.05) was used to compare positive p-STAT3 nuclei between cells expressing STAT3-GOF variants and WT-STAT3. Representative images are shown (Magnification 40 ×).