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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Immunol. 2018 Aug 20;201(7):1907–1917. doi: 10.4049/jimmunol.1800465

Figure 5: Nfkbid expression levels modulate thymic negative selection efficiency of diabetogenic NY8.3 clonotypic CD8+ T-cells.

Figure 5:

(A) Illustration of gating to enumerate DP and CD8+ thymocytes staining with the NY8.3 TCR specific Tet-NRPV7. Depicted profiles are for thymocytes from an NOD.NY8.3 control. (B) Numbers of NY8.3 TCR expressing DP and CD8+ thymocytes from 6-week-old female NOD.NY8.3 and NOD.NY8.3-Nfkbid+/− heterozygous mice. Data represent the mean±SEM of DP or CD8+ SP thymocytes staining positive with the NY8.3 specific Tet-NRPV7 reagent. (C) Numbers of NY8.3 TCR expressing DP and CD8+ SP thymocytes from 4-week-old female NOD.NY8.3 and NOD.NY8.3-Nfkbid−/− homozygous mice. Bars represent the mean±SEM of 3 independent experiments. (D) Geometric MFI of Tet-NRPV7 staining of DP and CD8+ SP thymocytes from NOD.NY8.3, NOD.NY8.3-Nfkbid+/−, and NOD.NY8.3-Nfkbid−/− mice. Bars represent mean±SEM. Statistical significance was analyzed by 2-way ANOVA and Bonferroni’s multiple comparison test. ns p>0.05, ** p<0.01.