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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Rev Cardiol. 2018 Oct;15(10):631–647. doi: 10.1038/s41569-018-0046-4

Figure 3. Ex vivo epicardial regeneration.

Figure 3.

(A) Whole-mount images of explanted zebrafish heart showing epicardial regeneration along the ventricular surface in a base-to-apex direction (arrows). tcf21:nucEGFP visualizes epicardial cell nuclei (green). dpi, days post Mtz incubation. (B) The epicardium generates a leading edge of large, multinucleate (leader, bottom) cells during migration ex vivo. Trailing (follower, top) cells are small and mononucleate. Epicardial nuclei are indicated in violet (tcf21:nucEGFP) and phosphorylated myosin light chain 2 (Ser19), an indicator of mechanical tension, is cyan.