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The HPLC chromatograms of reactions containing 50 μM of MftA variant with and without 50 μM MftE, indicate that MftE preferentially hydrolyses MftA* to form two new products at retention times 12.5 min and 13.9 min (orange). All reactions were carried out in the presence of 50 μM Zn2+ and 50 μM Fe2+.
