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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Mol Neurobiol. 2018 Mar 18;55(11):8438–8454. doi: 10.1007/s12035-018-1004-1

Figure 6. DMR induced by NE, DA and synthetic DA receptor ligands using label-free assay.

Figure 6

DMR assay was performed in CHO cells transfected with α2A adrenoceptor. (A) Cells were pretreated (dotted lines) or not (full lines) with the α2A adrenoceptor antagonist BRL 44408 at 1 μM for 30 minutes before adding the endogenous agonists DA or NE at 300 nM or 1 μM. (B) Cells were treated with 100 nM of several synthetic DA receptor ligands. The resulting shifts of reflected light wavelength (pm) were monitored over time. Each panel is a representative experiment of n = 3 different experiments. Each curve is the mean of a representative optical trace experiment carried out in triplicates.