Fig. 3.

BCG-induced antigen-specific CD4⁺ T cells display a dominance of multifunctional cells. Following BCG immunisation, intravascular staining and ICS identified populations of antigen-specific (cytokine⁺) CD4⁺ T cells in the lungs, spleen and peripheral blood producing IFN-γ, IL-2 or TNF-α alone or in combination. Graphs show frequencies of BCG-induced antigen-specific CD4⁺ T cells in all compartments as a % of the total CD4⁺ T cells in the same compartment at week 5 post-BCG vaccination. Data for all other time points is presented in Supplementary Fig. 2. For all graphs, bars represent mean ± SEM (n = 6). Two-way ANOVA with Sidak’s post-test, comparing BCG and control, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001.