Fig. 4.
BCG-induced antigen-specific CD4+ T cells display an effector phenotype. Following BCG immunisation, intravascular staining and ICS identified populations of antigen-specific (cytokine+) CD4+ T cells. (a) Representative plots showing surface staining for CD62L and CD27 on antigen-specific CD4+ T cells from a mouse 5 weeks post-BCG vaccination. (b) Frequency of antigen-specific CD4+ CD44hi T cells in all compartments displaying combinations of CD62L and CD27 cell surface markers as a % of total CD4+ T cells in that compartment at week 5 post-vaccination. Data for all other time points is presented in Supplementary Fig. 3. Bars represent mean ± SEM (n = 6). Two-way ANOVA with Sidak’s post-test, comparing BCG and control, ****P < 0.0001.