Fig. 5.

BCG-induced antigen-specific CD4⁺ T cells are CCR7⁻ but heterogeneous for CD69 and CD127. Following BCG immunisation, intravascular staining and ICS identified populations of antigen-specific (cytokine⁺) CD4⁺ T cells. (a) Representative plots showing surface staining for CCR7, CD69 and CD127 on antigen-specific CD4⁺ T cells from a mouse 5 weeks post-BCG vaccination. (b) Frequency of antigen-specific CD4⁺ CD62L^lo T cells in all compartments displaying CCR7, CD69 and CD127 cell surface markers as a % of total CD4⁺ T cells in that compartment at week 5 post-BCG vaccination. Data for all other time points is represented in Supplementary Fig. 4. For all graphs, bars represent mean ± SEM (n = 6). Two-way ANOVA with Sidak’s post-test, comparing BCG with control and BCG with BCG, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. (c) Median fluorescence intensity (MFI) of antigen-specific CD4⁺ CD69⁺ populations in the lung parenchyma and lung vasculature at week 5 post-vaccination. Data for all other time points is represented in Supplementary Fig. 5. Lines represent mean ± SEM (n = 6). Unpaired two-tailed t-test, ^**P < 0.01.