Figure 2.

Levels of CoQ and the biosynthetic proteins COQ7, COQ5, COQ6 and COQ8A. (A) CoQ₉ levels in liver homogenates of young adult, middle-age and old Coq9^Q95X and Coq9^+/+ male mice. (B–E) Representative western blot of COQ7 (B), COQ5 (C), COQ6 (D) and COQ8A (E) and their quantitative analysis in the liver of young adult, middle-age and old Coq9^Q95X and Coq9^+/+ male mice. *P < 0.05; **P < 0.01; *** P < 0.001; Coq9^Q95X versus Coq9^+/+ littermates mice. The mitochondrial Tom20 was used as loading control. The specific band for each target protein is identified with an arrow, and only these bands were used in the quantification (the lower bands in COQ5 and COQ6). Note that the anti-COQ6 antibody recognizes two bands that may correspond to the full-length and mature (mitochondrial import) forms of COQ6 or, alternatively, to two different COQ6 isoforms^38,39. Data information: (A–E) Data are expressed as mean ± SD. Statistical analyses were performed on Coq9^+/+ male mice versus Coq9^Q95X male mice by Multiple Student’s t-test one per row. (A) Coq9^+/+ mice, n = 6; Coq9^Q95X mice, n = 6, at each age (B–E) Coq9^+/+ mice, n = 5; Coq9^Q95X mice, n = 5, at each age WB images from (A–E) were cropped from two different parts of the same gel at the same exposure.