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. 2018 Sep 18;8:14013. doi: 10.1038/s41598-018-32190-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inmunohistochemistry against 8-OHdG as a marker of oxidative damage. (A–D) Representative negative control of the liver at 3–3.5 month of age in Coq9^+/+ (A) and Coq9^Q95X mice (B); and at 18–20 month of age in Coq9^+/+ (C) and Coq9^Q95X (D) mice. (E–H) Representative anti-8OHdG immunohistochemistry of the liver at 3-3.5 month of age in Coq9^+/+ (E) and Coq9^Q95X mice (F); and at 18–20 month of age in Coq9^+/+ (G) and Coq9^Q95X (H) mice. Data information: Scale bars: 100 µm. (I) Percentage of 8OHdG positive signal in the images. Results correspond to the mean ± SD, as determined by the software ImageJ. Coq9^+/+ mice n = 3; Coq9^Q95X mice n = 3, at each age.