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. 2018 Feb 14;25(9):1612–1624. doi: 10.1038/s41418-018-0065-z

Fig. 1.

Fig. 1

Pin1 promotes cell growth, migration and invasion in HCC cells. a Western blotting and b quantification of Pin1 expression in human HCC tumors and their adjacent normal tissues. CB: Coomassie blue staining. c, f, i, j Pin1 expression measured by western blotting in SK-Hep1-stable transfectants c or Huh-7 MEKDD stable transfectants f expressing shPin1 and shCtrl, in LM3, SK-Hep 1, and Huh-7 cells i, or in LM3 cells with or without Pin1 overexpression j. d, g, k MTT assays for SK-Hep1 shPin1 and shCtrl cells d, Huh7 MEKDD shPin1 and shCtrl cells g, or LM3 Pin1 and LM3 Ctrl cells k. e, h, l Transwell migration and invasion assays for SK-Hep1 shPin1 and shCtrl cells e, Huh7 MEKDD shPin1 and shCtrl cells h, or LM3 Pin1 and LM3 Ctrl cells l. Error bars indicate ± SD. Statistical significance was determined by the Student’s t-test. *P < 0.01. Experiments were repeated three times.