Fig. 4.

Tmco1 ablation results in abnormal Ca²⁺ signaling and ER Ca²⁺ overload in GCs. Black arrows indicated the time of treatment. a Ionomycin (1 μm)-evoked Ca²⁺ transients in primary GCs in Ca²⁺-free medium. b Histogram of the average peak area of ionomycin-evoked Ca²⁺ transients in GCs. c TG (1 μm)-triggered Ca²⁺ signaling in GCs. d Bar chart presented the average of peak area of TG-induced Ca²⁺ transients. e Representative curves of ATP (100 μm)-stimulated Ca²⁺ response. f Summarized bar graph depicted much more elevation of Ca²⁺ mobilization induced by ATP in KO GCs