Fig. 5.

RNA-seq and IPA show altered diseases and functions in Tmco1^−/− ovaries. a Diseases and biological functions analysis of DEGs (FC > 1.5) identified by IPA. b qRT-PCR assays were used to validate the DEGs predicted by RNA-seq. RNA was isolated from 7-day-old mouse ovaries of Tmco1^+/+ (N = 5) and Tmco1^−/− mice (N = 5). Six upregulated genes and four downregulated genes were determined and normalized by mouse Gapdh gene. c−e Relative mRNA levels were performed by qRT-PCR in GCs isolated from 23-day female mice after 48 h administration of PMSG (N = 6). c DEGs predicted by RNA-seq and d genes associated in follicular development as well as e calcium signaling were examined. The expression levels of Lgals3 (*p = 0.0208), Igf2 (*p = 0.0163), Mmp9 (**p = 0.0039), and Myh11(*p = 0.0169) were significantly increased in Tmco1^−/− GCs; nevertheless, the expression levels of Esr2 (*p = 0.0160), Inhbb (**p = 0.0049), Cyp19a1 (*p = 0.0352), Inha (*p = 0.0337) and Inhba (*p = 0.0244) were decreased in Tmco1^−/− GCs