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. 2018 Sep 18;8:13964. doi: 10.1038/s41598-018-31889-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of percentages of monocyte subpopulations and expression of surface markers on monocytes from healthy donors and dialysis patients. Blood from healthy donors (CON) and hemodialysis patients (HD) before (pre) and after (post) a dialysis session was stained with fluorochrome labeled antibodies to gate for monocyte subsets as outlined in supplementary Fig. 1. The scatter plots show the percentages of Mo1, Mo2 and Mo3 of total monocytes (A), the expression of CD11b (B), CD18 (C), CD36 (D), CD163 (E), TLR2 (F), CCR2 (G) and CX3CR1 (H) from 16 healthy controls (blank circles) and 15 HD patients before (HD pre, black circles) and after (HD post, black diamonds) a dialysis session. P-values comparing CON with HD pre or HD post were calculated with Mann-Whitney U-test, p-values comparing HD pre and HD post with Wilcoxon matched-pairs signed rank test. MFI: median fluorescence intensity. n.s.: not significant.