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. 2018 Sep 18;8:13964. doi: 10.1038/s41598-018-31889-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Response of healthy controls’ or HD patients’ monocytes upon LPS stimulation Whole blood from healthy donors (CON, n = 7) and HD patients (HD, n = 9, collected both before, pre, and after, post, a single dialysis session) was incubated for 30 min with 10 ng/ml LPS or left untreated. The graphs show the percentual change of TLR2 (A), TLR4 (B), CD18 (C) or CD11b activated (D) expression on all three monocyte subpopulations in healthy volunteers (white bars), or HD patients before (grey bars) and after (black bars) a single dialysis session. P-values comparing CON with HD pre or HD post were calculated with two-tailed Mann-Whitney U-test, p-values comparing HD pre and HD post with Wilcoxon matched-pairs signed rank test. MFI: median fluorescence intensity.