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. 2018 Sep 18;9:3804. doi: 10.1038/s41467-018-06225-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — CSF-contacting neurons require Pkd2l1 to respond to mechanical pressure. a Transmitted light and b fluorescent images of a cultured CSF-cN from a Tg(pkd2l1:TagRFP) embryo. Scale: 5 µm. c Gap-free VC recordings from CSF-cNs in vitro show extensive single channel opening in pkd2l1^+/+ but not pkd2l1^−/− embryos, comparable to results in vivo. Scale: 2 s, 20 pA. d Gap-free VC recording from a cultured CSF-cN while a mechanical stimulus is applied, showing an increase in channel opening during the stimulus in pkd2l1^+/+. Scale: top: 20 ms, 25 pA, bottom: 20 ms, 2 µm. e Gap-free recording from cultured CSF-cNs while a mechanical stimulus is applied. Scale: top: 20 ms, 25 pA, bottom: 20 ms, 2 µm. f Quantification of channel opening probability in response to mechanical stimulation of pkd2l1^+/+ and pkd2l1^−/− CSF-cNs (n = 7 pkd2l1^+/+ CSF-cNs, pre vs. stim p = 1.6 × 10^–6; n = 10 pkd2l1^−/− CSF-cNs, pre vs. stim p = 0.76, paired t test). Error bars represent s.e.m.