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. 2018 Sep 18;9:3804. doi: 10.1038/s41467-018-06225-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Pkd2l1 is necessary to maintain a straight spine. a Representative pkd2l1^+/+ and sibling pkd21^−/− adult zebrafish at adult stages. Scale: 1 cm. b Body curvature traces from pkd2l1^+/+ (black) and pkd2l1^−/− (blue) adults at 12 months (n = 11 pkd21^+/+, n = 11 pkd2l1^−/−). c Quantification of body axis curvature. Each point represents one fish. Error bars represent s.e.m. (5 months: n = 14 pkd2l1^+/+, n = 13 pkd2l1^−/−, p = 0.02; 12 months: n = 11 pkd2l1^+/+, n = 11 pkd2l1^−/−, p = 1.42 × 10^‒4; 19 months: n = 7 pkd2l1^+/+, n = 11 pkd2l1^−/−, p = 1.5 × 10^‒3; two-sample t test). d Alizarin red staining of bones of an adult pkd2l1^+/+ fish at 20 months of age, showing little to no spinal kyphosis. Scale: 5 mm. e High-magnification image of the precaudal region of the pkd2l1^+/+ spine. Dotted lines indicate vectors used to calculate the Cobb angle. Scale: 1 mm. f Alizarin red staining of an adult pkd2l1^−/− fish. Note the kyphosis in the precaudal region. Scale: 5 mm. g High-magnification image reveals pronounced kyphosis, no torsion is evident (see Supplementary Table 2 for Cobb angles). Scale: 1 mm