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. 2018 Sep 18;8:13979. doi: 10.1038/s41598-018-32334-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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15d-PGJ₂-loaded NC inhibit the oxidative stress induced by MSU in a PPAR-γ-dependent manner. Fifteen hours after MSU, knee joint was collected for the determination of oxidative stress by measuring GSH levels (A), FRAP assay (B), ABTS assay (C), superoxide anion prodution (NBT assay) (D), NO production (NO₂⁻ assay) (E), and determination of the mRNA expression for gp91phox (F), Nrf2 (G), and Ho-1 (H) by RT-qPCR. Results are mean ± SEM, n = 6 mice per group in each experiment, two independent experiments (*p < 0.05 vs. control group; ^#p < 0.05 vs. vehicle mg/kg group, **p < 0.05 vs 15d-PGJ₂-loaded NC. One-way ANOVA and post-test of Tukey).