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. 2018 Sep 18;8:14012. doi: 10.1038/s41598-018-32356-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SAM/SAH and adenosine measurements. (A) Levels of SAM and SAH (ng/ml) and their ratio (SAM/SAH) in the lysates of AHCY-silenced and control cells, as measured by LC-MS/MS. ± SD is represented as vertical line and is based on three independent measurements. (B) Adenosine was determined in the deproteinised cell lysates of AHCY-knockdown and control cells using an Adenosine Assay kit (BioVision) and represented as nmole adenosine per mg of total protein in the cell lysate. Vertical lines represent ± SD of two 2 individual measurements performed in triplicates for each sample (*means P < 0.05; determined by two tailed t-test).