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. 2018 Sep 18;8:14012. doi: 10.1038/s41598-018-32356-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphometric and cell cycle analysis. (A) Effects of AHCY silencing on the cell cycle distribution determined by flow cytometry after staining with propidium iodide. G1, S and G2/M indicate the cell phase in question. The graphs show the mean ± SD of three independent experiments performed in triplicate for each tested sample. (B) Changes in the cell proliferation rates of AHCY-silenced cells compared to control cells, as determined by NRA and MTT tests. The graphs show the mean ± SD of three independent experiments performed in at least 10 wells/cell line (* means P < 0.0.05; determined by two tailed t-test). (C) Upper panel: representative nuclei images (blue: DAPI). Centered is a field of view containing >10 cells for each condition. Graph: changes in the main nuclear morphometric features in % when compared with shCTRL set as 100% (mean of more than 100 nuclei; ** means p < 0.01 determined by two tailed t-test). Imaged with a Leica SP8 X FLIM confocal microscope (HC PL APO CS2 63 ×/1.40 OIL objective); scale bar, 50 µm.