Figure 3.

MEK and γ-Secretase are among targets identified for ROS shedding regulation. (a) The shedding distance, D^S, was measured as the distance from the mCherry^TM stripe to the tip of the ROS and represents the growth that occurred before heat shock minus the amount shed during compound treatment. (b) Signaling pathways targeted by compounds that increased D^S, and therefore suppressed shedding. (c) Two MEK5 inhibitors increased D^S as compared to DMSO control (0.2%). Both MEK5 inhibitors were screened during the same week and compared to the same DMSO control. (d) Signaling pathways targeted by compounds that decreased D^S, and therefore promoted shedding. (e) γ-Secretase inhibitors increased D^S as compared to DMSO control (0.2%). Inhibitors YO-01027 and LY411575 were screened during the same week and compared to the same DMSO control. Each ROS D^S measurement is represented by a small dot of color that represents an individual fish (n = 24–105 rods from 3 fish). Lower and upper hinges of box plots correspond to first and third quartiles; middle line corresponds to median; whiskers extend 1.5 * interquartile range above and below the hinges; large single black dot represents the mean; small black dots represent outliers. ***p < 0.001; **p < 0.01, *p < 0.05 by Dunnett’s comparison.