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. 2018 Feb 19;25(9):1598–1611. doi: 10.1038/s41418-018-0064-0

Fig. 8.

Fig. 8

Downregulation of GATA4 restores the axon regeneration phenotype exhibited by Cbx7 knockdown in adult DRG neurons. a Comparison of GATA4 expression level in cultured DRG sensory neurons between DIV0 and DIV3 (n = 6, p < 0.01). b Gata4 mRNA level was tested after 3 days of GATA4 electroporation into DRG sensory neurons (n = 3, *p < 0.05). The Gata4 level was quantified using qRT-PCR and normalized to the expression of the GAPDH. c Adult DRG neurons were transfected with GFP siRNA ctrl and siRNAs against Gata4 (siGata4) as indicated. At 3 days after transfection, neurons were fixed and immunostained with anti-GFP and anti-TuJ1 antibodies. Representative images of neurons transfected with siRNAs and GFP are shown. d Quantification of axon length of adult DRG neurons transfected with GFP plus siRNA ctrl or siGata4 (n = 148 for control, n = 185 for siGATA4) (n = 3, *P < 0.05). Scale bar, 100 µm. e Downregulation of Gata4 could restore the increased axon length of adult DRG caused by downregulation of CBX7 as measured GFP and Tuj1 positive cells. Representative images are shown. Scale bar, 100 µm. f Quantification of axon length of adult DRG neurons transfected with GFP, siCbx7, siGata4, or siCbx7 plus siGata4 (n = 82 for control, n = 74 for siCbx7, n = 70 for siGata4, and n = 48 for siCbx7 + siGata4) (n = 4 repeats, *p < 0.05)