Fig. 6.

MamiRs target JNK/MAPK signaling biologically and attenuated its activity. a, c, e Western blotting analysis shows the expression of JNK/MAPK signaling protein (JNK, c-Jun, MKK7, and MKK4) and their phosphorylated forms (p-JNK, p-c-JUN, p-MKK7, and p-MKK4) in C2C12 cells that were transfected with mamiR mimics or NC. b, d, f Relative expression in a, c, and e were calculated, respectively. GAPDH was the internal control. g Western blotting analysis shows the protein expression of key factors of JNK/MAPK signaling (MEKK1, MEKK2, p-JNK, JNK, p-c-Jun, and c-Jun) in C2C12 cells that were transfected with mamiR inhibitors or NC inhibitor. h Relative expression in g was calculated. eThe pathway assay revealed mamiRs affected the activity of JNK/MAPK and P38/MAPK signaling. The results are normalized to those of with NC. Values are means ± SEM. The statistical significance of difference between two means was calculated with the t-test, *p < 0.05; **p < 0.01