Fig. 7.

mamiRs promote myogenesis and form an autoregulatory loop with JNK/MAPK and P38/MAPK signaling. a Immunofluorescent microscopy analysis of the morphological changes and expression of p-c-Jun and myogenesis markers in C2C12 cells transfected with mamiR mimics or NC. bMHC⁺ cells were calculated based on staining described in panel a. c qRT-PCR assays of myogenic markers (MHC, MEF2C, and MyoD) in C2C12 cells transfected with mamiR mimics or NC. d Western blotting for myogenic markers (MHC, MEF2C, and MyoD) in C2C12 cells transfected with mamiR mimics or NC. e Relative expression in d were calculated. GAPDH was the internal control. f qRT-PCR assays of mamiR expression in C2C12 cells treated with SP600125, SB203580, or DMSO. The results are normalized with to those of cells treated with DMSO. Values are means ± SEM. The statistical significance of difference between two means was calculated with the t-test, *p < 0.05; **p < 0.01