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. 2018 Sep 18;9:3805. doi: 10.1038/s41467-018-05777-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Transmission-blocking efficacy of leads in P. falciparum SMFA. The predictive power of the Pf DGFA to identify transmission-blocking compounds was assessed by treating GFP/luciferase-expressing P. falciparum gametocytes for 24 h with DMSO, DDD291 or DDD599/BPCA and then feeding them to A. stephensi mosquitoes in indirect mode in which test compound is absent from the blood meal. As DDD881 targets gamete formation rather than gametocytes, membrane feeds with this compound were performed in direct mode, added directly to the blood meal with no prior incubation. a Normalised luciferase intensity from individual mosquitoes (n = 56–72 per condition per replicate) was compared to DMSO and unfed (uninfected) controls. Data combined from three independent biological replicate feeds. Grey dots indicate individual luciferase readout for individual mosquitoes and black bar shows the mean. b Calculated infection prevalence of mosquito feeds normalised to respective DMSO control. Error bars denote the standard error of the mean